PuzzlePage
Career, tech & modernGrades 11–12 / health science CTE15 cardsFact-checked

Phlebotomy Order of Draw Flashcards

The CLSI order of draw tube by tube: stopper colour, additive, what it is for, and the additive carryover each step is there to prevent. With the mnemonic.

Free to play in your browser — no account, no install. All 15 cards are free forever — the deck is shorter than the free 30. The deck opens with The CLSI order of draw, start to finish, First tube, Second tube and Third tube and runs to 15 cards in all.

Flip freely. Nothing is scored — go round as many times as you like.

Card 1 of 151 of 15 seen

Space flip · move · S shuffle · F flip deck · D dark

Phlebotomy Order of Draw flashcards — the free 15

Every question and answer on the free cards, written out. Check them against your own textbook before you spend anything.

The CLSI order of draw, start to finish
Blood culture, light blue, red or gold, green, lavender, gray
First tube
Blood culture bottle or tube — drawn before anything that could break its sterility
Second tube
Light blue — sodium citrate for coagulation tests; fill to the mark for the 9:1 blood-to-additive ratio
Third tube
Red or gold — serum, with or without clot activator and gel; chemistry, serology, drug levels
Fourth tube
Green — heparin, lithium or sodium, with or without gel; plasma chemistry
Fifth tube
Lavender or pink — EDTA; CBC and blood films, pink for blood bank work
Sixth tube
Gray — sodium fluoride with potassium oxalate, a glycolytic inhibitor; glucose and lactate
Why the order exists at all
To stop additive carrying over from one tube into the next and altering the result
What EDTA carryover does to a chemistry result
Falsely raises potassium and drops calcium — so EDTA tubes follow the chemistry tubes
Why the citrate tube goes before the serum tubes
Clot activator shortens PT and aPTT; only cultures or plain tubes may precede the coagulation tube
Why the gray tube goes last
Fluoride and oxalate distort cell shape on a differential, so lavender is drawn before gray
A mnemonic for the order
Sally Brings Really Good Grease and Leaves the Gravy — sterile, blue, red, gold, green, lavender, gray
Who sets the order of draw
CLSI — its venous blood collection standard GP41, 7th ed., republished as PRE02, 8th ed.
When a discard tube is needed
When the citrate tube comes first through a winged set — it fills the tubing's dead space
Order of draw for a skin puncture
Reversed — EDTA for hematology first, other additives next, serum last (CLSI GP42)

Phlebotomy Order of Draw flashcards — questions people ask

How many cards are in the Phlebotomy Order of Draw flashcard deck?
The Phlebotomy Order of Draw deck on PuzzlePage has 15 cards, and all 15 are free to play and free to download with no account and no payment. 15 cards. The order of draw is six tube groups long; the rest of the deck is the reason behind each step, the mnemonic, and the two cases that change the procedure. There is no honest way to make it longer.
What is on the phlebotomy order of draw flashcards?
The CLSI order of draw tube by tube: stopper colour, additive, what it is for, and the additive carryover each step is there to prevent. With the mnemonic. Cards look like this: The CLSI order of draw, start to finish → Blood culture, light blue, red or gold, green, lavender, gray; First tube → Blood culture bottle or tube — drawn before anything that could break its sterility. The deck is written for grades 11–12 / health science cte and filed under career, tech & modern.
Can I study phlebotomy order of draw flashcards online for free?
Yes. All 15 cards of the Phlebotomy Order of Draw deck play free in any web browser at puzzlepage.app, with no account, no app and no card details. It works on a phone, tablet, Chromebook, laptop or classroom projector. Practice mode flips through the cards with nothing scored; quiz mode makes you answer each card before it flips, times the run and scores it.
Are the phlebotomy order of draw flashcards accurate?
Every card in the Phlebotomy Order of Draw deck cites two independent published sources — two different organisations, not a standards body and a site reprinting it — and a script checks that, rather than taking the writer's word for it. A second specialist then re-verified the deck against sources of their own, without reading the citations first. That is the difference from a user-generated flashcard site, where the cards are whatever a stranger typed and nobody is accountable for them.
Can I print the phlebotomy order of draw flashcards?
Yes. The Phlebotomy Order of Draw cards are available as a printable double-sided PDF to cut up, as well as playable in the browser. The whole 15-card printable is free to download, with no account.
How should I use the Phlebotomy Order of Draw deck to revise?
Start in practice mode and go through the Phlebotomy Order of Draw deck once without scoring, to find out which cards you already know — the deck is dealt in a different order every time you open it, so you are recalling the card rather than its position. Then switch to quiz mode, which times and scores the run and lets you re-run only the cards you missed. Use the "flip deck" control to swap which side is the question: recognising an answer is much easier than producing one, so a deck you find easy one way round is usually the harder and more useful drill the other way. Two or three short sessions across a week beat one long one, which is the whole reason flashcards work.
What grade level are the phlebotomy order of draw flashcards for?
The Phlebotomy Order of Draw deck is written for grades 11–12 / health science cte. Because the cards are plain term-and-answer pairs, they are also used outside that band — by adults revising career, tech & modern, and by teachers running them as a whole-class starter on a projector.

More career, tech & modern decks

All 78 decksEvery ready-made deck on the site, free to start.Browse the shelfPrint this deck insteadThe same 15 cards as double-sided cards to cut up.See the printable